Tools that are mentioned include zinc finger nucleases zfns transcription activator like effector nucleases talens and crispr cas9 all of which have received much attention in recent years as breakthrough technologies genome editing with engineered nucleases allows us to precisely change the target genome of living cells and is a powerful way to control functional genes it is feasible in almost all organisms ranging from bacteria to plants and animals as well as in cultured cells . Targeted genome editing using site specific nucleases zfns talens and the crispr cas9 system amazonde yamamoto takashi fremdsprachige bucher 11899 eur preisangaben inkl ust abhangig von der lieferadresse kann die ust an der kasse variieren. Genome editing involves the use of four major classes of sequence specific nucleases ssns engineered homing endo nucleases or meganucleases zinc finger nucleases zfns transcription activator like effector nucleases talens and clustered regularly interspaced short palindromic repeats crispr cas9 these ssns direct the repair of the dna of double strand breaks dsbs at sites where a dna sequence modification is desired the repair is initiated either through non homologous end . Applications of genome editing tools are then described in detail in ips cells and diverse organisms such as mice rats marine invertebrates fish frogs and plants tools that are mentioned include zinc finger nucleases zfns transcription activator like effector nucleases talens and crispr cas9 all of which have received much attention in recent years as breakthrough technologies genome editing with engineered nucleases allows us to precisely change the target genome of living . Targeted genome editing using site specific nucleases zfns talens and the crispr cas9 system english edition ebook yamamoto takashi amazonde kindle shop